Search results for "Mono mac 6"

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Characterization of a Computerized Assay for Rapid and Easy Determination of Leukocyte Adhesion to Endothelial Cells

2005

We report on a facile and rapid computerized in-vitro assay for the quantification of leukocyte adhesion to endothelial cells under static conditions using bovine polymorphonuclear neutrophils (PMN) or human leukaemic Mono Mac 6 cells (MM6) and bovine aorta endothelial cells (BAEC). Images of leukocytes adherent to BAEC monolayers grown in microtiter plates were obtained by a digital camera attached to a conventional microscope and transferred to the public domain NIH ImageJ program for analysis. Using individually adapted program routines adherent leukocytes are easily discriminated and reproducibly quantified. The results obtained with our assay correspond to previous findings and demonst…

Pathologymedicine.medical_specialtyNeutrophilsPharmaceutical ScienceBiologyCell LinePolymorphonuclear NeutrophilsCell AdhesionImage Processing Computer-AssistedmedicineAnimalsHumansCells CulturedBovine aortaPharmacologyMicroscopyEndothelial CellsMono mac 6General MedicineAdhesionMolecular biologyMechanism of actionCell cultureCattlemedicine.symptomSelectinBiological and Pharmaceutical Bulletin
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